160-words-for-your-materials-and-methods

1. Activated: The reaction was activated by incubation at 100 °C

2. Added: …5 mL NaOH was added to the solution

3. Adjusted: …and the pH was adjusted to 7.5

4. Air-dried: …and the samples were air-dried for 24 h

5. Allocated: The animals were randomly allocated to three groups

6. Amplified: DNA was amplified using Taq polymerase

7. Analyzed / analysed: All data was analysed using the Student’s t-test

8. Anesthetised /anesthetized: The rats were anesthetised using ketamine

9. Applied: …and primary antibody was applied

10. Assayed: Luciferase activity was assayed using the Dual Luciferase assay kit

11. Autoclaved: The solutions were autoclaved at 121 °C for 20 min

12. Baked: ….the slides were baked at 200 °C for 1 h

13. Blocked: The membrane was blocked in TBS-T containing 5% non-fat milk

14. Boiled: The sample was boiled at 95°C for 5 min

15. Caged: The rats were caged individually

16. Calculated: The chlorophyll content was calculated as described in [21].

17. Centrifuged: The sample was centrifuged at 13,000 rpm for 15 min

18. Chilled: … chilled at 4 °C for 24 h, ….

19. Classified: The patients were classified into two groups based on p53 expression

20. Coated: The slides were coated with 3% APES in methanol

21. Collected: The cells were collected by centrifugation

22. Combined: The samples were combined for analysis

23. Constructed: The pEDGT vector was constructed using site-directed mutagenesis

24. Containing: …, washed in PBS containing 1% Tween-20

25. Cooled: The reaction was cooled

26. Correlated: The levels of P53 expression were correlated with EGFR expression

27. Counterstained: …. and counterstained with haematoxylin.

28. Covered: …. and leaves were covered with foil

29. Created: The pEYTH plasmid was created by PCR

30. Cultured: E. coli were cultured overnight at 37 °C

31. Defined: Pre-diabetes was defined as an impaired glucose tolerance test in the last 6

months.

32. Denatured: The DNA was denatured at 94 °C for 15 min

33. Designed: Primers were designed to quantify p53 and EGFR

34. Detected: Antibody binding was detected using enhanced chemiluminescence

35. Determined: The rate of the reaction was determined as described by Smith et al.

36. Developed: The staining intensity was developed by incubation in 5% copper sulphate

solution

37. Dialysed / Dialyzed: The sample was dialysed against 2M NaCl

38. Diluted: The sample was diluted to a final volume of 10 ml

39. Discarded: The supernatant was discarded

40. Dissected: Tumours were dissected from the normal tissue

41. Dissolved: Ampicillin was dissolved in water

42. Donated: The plasmids and p53 antibody were donated by Prof. J Smith.

43. Dried: The leaves were dried

44. Driven: Gene expression was driven by the CMV promoter

45. Electrophoresed: The samples were electrophoresed on 12% SDS-PAGE gels

46. Eliminated: Patients with Stage IV breast cancer were eliminated from the survival

analysis

47. Elongated: The PCR product was elongated at 72 °C for 10 min

48. Embedded: The liver and kidney samples were embedded in paraffin

49. Estimated: Survival was estimated using the Kaplan-Meier method

50. Euthanized: The animals were euthanized after 24 days

51. Excised: HPRT was excised from the plasmid using Bam HI

52. Expressed: Human CRCX2 was expressed in HeLa cells

53. Extracted: DNA was extracted using the phenol:chloroform method

54. Extrapolated: The curve was extrapolated to determine the intersection of the y-axis

55. Fed: Animals were fed a high-fat diet

56. Filtered: The solution was sterile filtered at 0.2 µM

57. Fixed: The tissues were fixed in paraformaldehyde

58. Followed: …30 cycles of 94°c for 1 min, 60°c for 1 min and 72°c for 1 min, followed by

72°c for 15 min

59. Frozen: The samples were snap frozen in liquid nitrogen

60. Genotyped: The mice were genotyped using PCR

61. Graphed: The data was graphed using Microsoft Excel

62. Ground: The leaves were ground in a pestle and mortar

63. Grouped: The patients were grouped according to tumour stage

64. Harvested: Cells were harvested by scraping

65. Homogenized: The tissues were homogenised

66. Housed: Mice were housed individually under a 12 h light / dark cycle

67. Identified: Disease history was identified from medical records

68. Illuminated: The plants were illuminated using red light

69. Imaged: The sections were imaged by confocal microscopy

70. Immersed: Slides were immersed in fixative for 10 min

71. Incubated: Cells were incubated at 37 °C for 3 days

72. Indicated: The presence of fluorescent labelling indicated DNA damage

73. Infused: The drug was infused at 1 mL/min

74. Inhibited: The reaction was inhibited by addition of 2 mL of 1 M EDTA

75. Initiated: The reaction was initiated by addition of Taq Polymerase

76. Injected: The rats were injected with the drug or control saline solution

77. Inoculated: LB broth was inoculated with 100 µL bacterial supernatant and cultured for 24 h

78. Inserted: The FLOX gene was inserted downstream of the CMV promoter

79. Interrogated: The database was interrogated to identify miR-129 target genes

80. Interviewed: Patients were interviewed to collect a full medical history

81. Investigated: Gene expression was investigated using real-time PCR

82. Isolated: Plasmid DNA was isolated using standard methods

83. Labelled: Cells were labelled with a CD34 antibody

84. Loaded: The protein samples were loaded on 1% agarose gels

85. Localised / localized: Staining was localized to the nucleus

86. Lysed: Cells were lysed in RIPA buffer

87. Magnified: The samples were magnified for further analysis

88. Mated: Heterozygous male mice were mated with wild-type females

89. Measured: Gene expression was measured using real-time RT-PCR

90. Mixed: Developing solution was added, mixed and used for…

91. Modelled: The data was modelled using a system of partial differential equations

92. Neutralised / neutralized: The reaction was neutralised by addition of 5 mL of 2 M HCl

93. Normalised / normalized: The data was normalized to achieve a standard distribution

94. Observed: Cell migration was observed at 24 h, 48 h and 72 h

95. Obtained: Twenty six glioma samples were obtained from patients undergoing surgery

96. Pelleted: Cells were pelleted by centrifugation

97. Performed: Analysis was performed according to the method of Jones et al. (1996).

98. Picked: Individual colonies were picked using sterile loops

99. Plated: HeLa cells were plated in 30 mm-diameter dishes

100. Plotted: Data was plotted using SPSS software

101. Precipitated: The DNA was precipitated by addition of 15 mL isopropanol

102. Prepared: The samples were prepared as previously described (23).

103. Probed: Membranes were probed with DIG-labelled probes

104. Programmed: PCR was performed using a thermocycler programmed for …

105. Purchased: The CD34 antibody was purchased from Cell Signaling

106. Purified: The plant extract was purified using the method of Jones at al. (2005).

107. Quantified: The bands were quantified using the Gel Doc System

108. Queried: The database was queried using the following search terms:

109. Radio-labelled: Proteins were radio-labelled with P32

110. Reacted: After the enzyme and tissue had reacted, the solutions..

111. Received: All patients received MRI and CT scans

112. Recorded: The data was recorded in Excel

113. Recovered: The DNA was recovered by isopropanol precipitation

114. Recruited: Two hundred type 2 diabetes patients were recruited for this study

115. Removed: The leaves were removed 6 h after treatment

116. Represented: Using the formula of Jones et al., the gradient can be represented as x – y / t

117. Resected: The tumours were resected, then the patients…

118. Resuspended: HeLa cells were resuspended in serum-free media

119. Reverse-transcribed: Total RNA was reverse-transcribed to cDNA

120. Rinsed: The pellet was rinsed in PBS

121. Sampled: Five leaves were sampled from each tree at 3 h intervals

122. Saturated: The membranes were statured with 100% ethanol

123. Scored: Staining was scored as previously described

124. Scraped: The cells were scraped into lysis buffer

125. Sectioned: Tumour blocks were sectioned at 10 µm

126. Stained: Sections were stained using hematoxylin and eosin

127. Selected: Based on the pilot study, 200 mM cisplatin was selected for further experiments

128. Semi-quantitatively scored: Immunostaining was semi-quantitatively scored

129. Separated: Proteins were separated on using a SDS-PAGE gel

130. Shaved: The skin was shaved before surgery

131. Sized: Bands were sized using a DNA ladder

132. Soaked: The membrane was soaked in 100% methanol

133. Spread: Bacterial suspension was spread on LB agar plates

134. Staged: The patients were staged according to the WHO criteria

135. Standardised / Standardized: The assay was standardised by including the standard curve in each replicate

136. Sterilised / Sterilized: The slides were sterilised by baking at 200°C

137. Stirred: then 5 ml EDTA was added, stirred, and the solution was…

138. Stored: The solution was stored at room temperature

139. Stratified: The patients were stratified according to tumour stage

140. Subjected: Samples were subjected to Western blotting

141. Supplemented: DMEM media supplemented with 10% foetal calf serum

142. Sutured: Skin was sutured and the animals were allowed to recover

143. Swabbed: Skin was swabbed with 70% EtOH before surgery

144. Termed: The pcDNA-EGFR plasmid, termed pEGFR in this study,

145. Terminated: The reaction was terminated by addition of 5 ml EDTA

146. Tested: Our hypothesis was tested by investigating the …

147. Titrated: The solution was titrated against 2 M HCl

148. Transferred: Proteins were transferred to PVDF membrane,

149. Transformed: Data was transformed to achieve a normal distribution

150. Treated: Cells were treated with 0, 10, 20 and 30 mM 5-FU for 24 h

151. Trimmed: Frozen blocks were trimmed and sectioned

152. Undergoing: Normal tissues were obtained from patients undergoing tonsillectomy

153. Underwent: All patients received liver ultrasound and CT scans

154. Used: Leaf samples were used for DNA extraction

155. Viewed: Sections were viewed using a light microscope

156. Visualised / visualized: Staining was visualised using DAB

157. Vortexed: The solution was vortexed for 30 s

158. Washed: Sections were washed in TBS-T

159. Weighed: Leaves were weighed

160. Wetted: The drug was wetted with EtOH before it was dissolved in water